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Resolution: standard / high Figure 3.
Real-time detection of the binding kinetics to the "GCG"-sequence immobilised on a glass fibre. Black curve corresponds to the interaction between matching pair of ZF1 and "GCG" motif (kD: 4,9·10-3 /s), the red curve corresponds to the ZF2 (kD: 7,3·10-3 /s) which should recognise the "AAA" sequence, but not the "GCG".
von Nickisch-Rosenegk et al. Journal of Nanobiotechnology 2005 3:5 doi:10.1186/1477-3155-3-5 |