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Resolution: standard / high Figure 4.
Real-time detection of the binding kinetics to the "AAA"-sequence immobilised on a glass fibre. Black curve corresponds to the interaction between matching pair of ZF2 and "AAA" motif (kD: 3,5·10-3 /s), the red curve corresponds to the ZF1 (kD: 4,6·10-3 /s) which should recognise the "GCG" sequence, but not the "AAA".
von Nickisch-Rosenegk et al. Journal of Nanobiotechnology 2005 3:5 doi:10.1186/1477-3155-3-5 |