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Resolution: standard / high Figure 5.
Fluorescence signal variation for successive prints. Variation of the fluorescence intensity for successive prints and for three different
contact times (15 seconds, 1 minute and 2 minutes) between the stamp and the slide.
Stamp was incubated with a 35-mer 5'-labelled Cy5 oligonucleotide for 30 sec than
put in contact with the dendrislides. The value of fluorescence intensity (fluorescent
– background) was measured at 635 nm with Genepix scanner under 600 PMT optical excitation.
Each point represents an average of 4 independent experiments. Fittings of the data
points with an exponential linear regression (solid lines), exhibits good agreement
as attested by the reported correlation factors R.
Thibault et al. Journal of Nanobiotechnology 2005 3:7 doi:10.1186/1477-3155-3-7 |