Figure 3.

Confocal imaging of Sf9 cells infected with recombinant baculoviruses. (A) AcEGFP-VP2, (B) AcEGFP-VP2-14, (C) AcEGFP-VP2-23, (D) AcEGFP-VP2-40, (E) AcVP2, (F) AcVP2-14, (G) AcVP2-23, and (H) AcVP2-40. A monoclonal anti-capsid antibody (A4E3) for detection of assembled VP2 was visualized with AlexaFluor^® 633-conjugated anti-mouse secondary antibody (violet), whereas EGFP (green) was imaged directly. Co-localization of the fusion partners in the merged images is shown in white. All images are single confocal midsections from single cells of approximately 0.7 μm in thickness. Bars 2 μm.