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Mobility of fluorescent canine parvovirus recombinant proteins. |
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| Particle |
τD1 (ms) |
τD2 (ms) |
D1 (m2s-1) |
No urea, RhD1 (nm) |
6 M urea, RhD2 (nm) |
Nf |
|
|
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| EGFP-VP2 |
0.7 ± 0.3 |
0.3 ± 0.1 |
1.4 × 10-11 |
17.0 ± 7.0 |
8.5 ± 2.7 |
9.5 ± 0.7 |
| EGFP-VP2-14 |
0.3 ± 0.0 |
0.2 ± 0.1 |
3.6 × 10-11 |
6.7 ± 0.9 |
3.9 ± 2.3 |
2.0 ± 0.1 |
| EGFP-VP2-23 |
0.8 ± 0.2 |
0.4 ± 0.1 |
1.2 × 10-11 |
20.0 ± 4.0 |
10.0 ± 2.5 |
5.8 ± 1.3 |
| EGFP-VP2-40 |
0.5 ± 0.1 |
0.4 ± 0.0 |
1.8 × 10-11 |
14.0 ± 3.4 |
9.0 ± 0.7 |
9.7 ± 0.1 |
| EGFP |
0.1 ± 0.0 |
0.1 ± 0.0 |
1.2 × 10-10 |
2.0 ± 0.1 |
2.0 ± 0.1 |
1.0 ± 0.0 |
|
The diffusion times before (τD1) and after (τD2) treatment with 6 M urea at 50°C, diffusion coefficient (D1), hydrodynamic radii in the absence (RhD1) and presence (RhD2) of 6 M urea at 50°C, as well as, the number of fluorescent moieties (Nf) present in the EGFP-VP2, EGFP-VP2-14, EGFP-VP2-23 and EGFP-VP2-40 fluorescent proteins and VLPs. The enhanced green fluorescent protein (EGFP) served as a reference. | ||||||
Gilbert et al. Journal of Nanobiotechnology 2006 4:13 doi:10.1186/1477-3155-4-13 |
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