Research

Evaluation of the microbial growth response to inorganic nanoparticles

Darryl N Williams , Sheryl H Ehrman and Tracey R Pulliam Holoman

Department of Chemical and Biomolecular Engineering, University of Maryland, 2113 Building 090, College Park, MD 20742, USA

author email corresponding author email

Journal of Nanobiotechnology 2006, 4:3doi:10.1186/1477-3155-4-3

Published:	28 February 2006

Abstract

In order to enhance the utilization of inorganic nanoparticles in biological systems, it is important to develop a fundamental understanding of the influence they have on cellular health and function. Experiments were conducted to test silica, silica/iron oxide, and gold nanoparticles for their effects on the growth and activity of Escherichia coli (E. coli). Transmission electron microscopy (TEM) and dynamic light scattering (DLS) were used to characterize the morphology and quantify size distribution of the nanoparticles, respectively. TEM was also used to verify the interactions between composite iron oxide nanoparticles and E. coli. The results from DLS indicated that the inorganic nanoparticles formed small aggregates in the growth media. Growth studies measured the influence of the nanoparticles on cell proliferation at various concentrations, showing that the growth of E. coli in media containing the nanoparticles indicated no overt signs of toxicity.