Research

Regulated growth of diatom cells on self-assembled monolayers

Kazuo Umemura^1,2 , Tomoaki Yamada² , Yuta Maeda² , Koichi Kobayashi² , Reiko Kuroda^3,4 and Shigeki Mayama⁵

¹  Kamoshita Planning, SP1112-5-15-1, Ginza, Chuo-ku, Tokyo 104-8238, Japan

²  Musashi Institute of Technology, 1-28-1 Tamazutsumi, Setagaya, Tokyo 158-8557, Japan

³  The University of Tokyo, 3-8-1 Komaba, Muguro-ku, Tokyo 153-8902, Japan

⁴  Kuroda Chiromorphology Project, ERATO-SORST, 4-7-6 Park Building, Komaba, Meguro-ku, Tokyo 153-0041, Japan

⁵  Tokyo Gakugei University, Koganei, Tokyo 184-8511, Japan

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Journal of Nanobiotechnology 2007, 5:2doi:10.1186/1477-3155-5-2

Published:	23 March 2007

Abstract

We succeeded in regulating the growth of diatom cells on chemically modified glass surfaces. Glass surfaces were functionalized with -CF₃, -CH₃, -COOH, and -NH₂ groups using the technique of self-assembled monolayers (SAM), and diatom cells were subsequently cultured on these surfaces. When the samples were rinsed after the adhesion of the diatom cells on the modified surfaces, the diatoms formed two dimensional arrays; this was not possible without the rinsing treatment. Furthermore, we examined the number of cells that grew and their motility by time-lapse imaging in order to clarify the interaction between the cells and SAMs. We hope that our results will be a basis for developing biodevices using living photosynthetic diatom cells.