Nanocurcumin downregulates steady state transcripts of multiple pro-inflammatory cytokines. Peripheral blood mononuclear cells (PBMCs) were activated with phytohaemagglutinin (PHA) and 1 μg/ml lipopolysaccharide (LPS), and exposed to either free curcumin or nanocurcumin at 20 μM. Thereafter, transcript levels of three pro-inflammatory cytokines IL-6, IL-8 and TNFα were measured by real time quantitative RT-PCR analysis. For IL-6, semi-quantitative RT-PCR was also performed to confirm the real time data. IL-6 expression is completely abrogated by exposure to free curcuminand nanocurcumin (A). IL-8 levels are also reduced with nanocurcumin therapy, albeit less than in free curcumin-treated PBMCs (B), while comparable and significant knockdown of TNFα transcripts is seen with both formulations(C). The error bars represent mean and standard deviations of experiments performed in triplicate.
Bisht et al. Journal of Nanobiotechnology 2007 5:3 doi:10.1186/1477-3155-5-3