Research

N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging

Shanta R Bhattarai¹ , Remant B Kc² , Sun Y Kim³ , Manju Sharma³ , Myung S Khil⁴ , Pyoung H Hwang , Gyung H Chung⁵ and Hak Y Kim⁴

¹  Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI, 48202 USA

²  Department of Bionanosystem Engineering, Chonbuk National Univiversity, Jeonju, South Korea

³  Deparrtment of Pediatric and Clinical Research Center, School of Medicine, Chonbuk National Univiversity, Jeonju, South Korea

⁴  Department of Textile Engineering, Chonbuk National Univiversity, Jeonju, South Korea

⁵  Department of Radiology, School of Medicine, Chonbuk National Univiversity, Jeonju, South Korea

author email corresponding author email

Journal of Nanobiotechnology 2008, 6:1doi:10.1186/1477-3155-6-1

Published:	4 January 2008

Abstract

This project involved the synthesis of N-hexanoyl chitosan or simply modified chitosan (MC) stabilized iron oxide nanoparticles (MC-IOPs) and the biological evaluation of MC-IOPs. IOPs containing MC were prepared using conventional methods, and the extent of cell uptake was evaluated using mouse macrophages cell line (RAW cells). MC-IOPs were found to rapidly associate with the RAW cells, and saturation was typically reached within the 24 h of incubation at 37°C. Nearly 8.53 ± 0.31 pg iron/cell were bound or internalized at saturation. From these results, we conclude that MC-IOPs effectively deliver into RAW cells in vitro and we also hope MC-IOPs can be used for MRI enhancing agents in biomedical fields.