Research

The margination propensity of spherical particles for vascular targeting in the microcirculation

Francesco Gentile¹ , Antonio Curcio² , Ciro Indolfi² , Mauro Ferrari^3,4 and Paolo Decuzzi^1,3

¹  Center of Bio-/Nanotechnology and -/Engineering for Medicine University of Magna Graecia at Catanzaro, Viale Europa – Loc. Germaneto, 88100, Catanzaro, Italy

²  Division of Cardiology, University of Magna Graecia at Catanzaro Viale Europa – Loc. Germaneto, 88100, Catanzaro, Italy

³  The University of Texas Health Science Center Houston 1825 Pressler St, Houston, Texas, 77030, USA

⁴  M.D. Anderson Cancer Center and Rice University 1825 Pressler St, Houston, Texas, 77030, USA

author email corresponding author email

Journal of Nanobiotechnology 2008, 6:9doi:10.1186/1477-3155-6-9

Published:	15 August 2008

Abstract

The propensity of circulating particles to drift laterally towards the vessel walls (margination) in the microcirculation has been experimentally studied using a parallel plate flow chamber. Fluorescent polystyrene particles, with a relative density to water of just 50 g/cm³comparable with that of liposomal or polymeric nanoparticles used in drug delivery and bio-imaging, have been used with a diameter spanning over three order of magnitudes from 50 nm up to 10 μm. The number of particles marginating per unit surface have been measured through confocal fluorescent microscopy for a horizontal chamber, and the corresponding total volume of particles has been calculated. Scaling laws have been derived as a function of the particle diameter d. In horizontal capillaries, margination is mainly due to the gravitational force for particles with d > 200 nm and increases with d⁴; whereas for smaller particles increases with d³. In vertical capillaries, since the particles are heavier than the fluid they would tend to marginate towards the walls in downward flows and towards the center in upward flows, with increasing with d^9/2. However, the margination in vertical capillaries is predicted to be much smaller than in horizontal capillaries. These results suggest that, for particles circulating in an external field of volume forces (gravitation or magnetic), the strategy of using larger particles designed to marginate and adhere firmly to the vascular walls under flow could be more effective than that of using particles sufficiently small (d < 200 nm) to hopefully cross a discontinuous endothelium.