Research

Quantum dots improve peptide detection in MALDI MS in a size dependent manner

Julian Bailes¹ , Loïc Vidal² , Dimitri A Ivanov² and Mikhail Soloviev¹

¹  School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

²  IS2M, CNRS LRC7228, 15, rue Jean Starcky, 68057 Mulhouse, France

author email corresponding author email

Journal of Nanobiotechnology 2009, 7:10doi:10.1186/1477-3155-7-10

Published:	31 December 2009

Abstract

Laser Desorption Ionization Mass Spectrometry employs matrix which is co-crystallised with the analyte to achieve "soft ionization" that is the formation of ions without fragmentation. A variety of matrix-free and matrix-assisted LDI techniques and matrices have been reported to date. LDI has been achieved using ultra fine metal powders (UFMPs), desorption ionisation on silicon (DIOS), sol-gel assisted laser desorption/ionization (SGALDI), as well as with common MALDI matrices such as 2,5-dihydroxy benzoic acid (DHB), 3,5-dimethoxy-4-hydroxycinnamic acid (SA), α-cyano-4-hydroxycinnamic acid (CHCA) to name a few. A variety of matrix additives have been shown to improve matrix assisted desorption, including silicon nanowires (SiNW), carbon nanotubes (CNT), metal nanoparticles and nanodots. To our knowledge no evidence exists for the application of highly fluorescent CdSe/ZnS quantum dots to enhance MALDI desorption of biological samples. Here we report that although CdSe/ZnS quantum dots on their own can not substitute matrix in MALDI-MS, their presence has a moderately positive effect on MALDI desorption, improves the signal-to-noise ratio, peak quality and increases the number of detected peptides and the overall sequence coverage.