The analysis of MALDI spectra: mass spectra calibration and mass matching. (A) Automatic peak assignment for all spectra using the list of BSA/trypsin masses and the "Auto Assign" function of the "FlexAnalysis" software. Vertical axis indicates the number of peaks automatically assigned in each case (mean +/- STDEV, from 3 experiments). (B) Minimum laser power setting for each matrix/QD preparation (from each individual experiment), at which automatic calibration was possible (mean +/- STDEV, from 3 experiments). (C) the highest number of BSA masses identified by MASCOT http://www.matrixscience.com webcite and the "mascot scores" (D) averaged over all spectra for each individual matrix/QD sample and shown as mean +/- STDEV, from 3 experiments.
Bailes et al. Journal of Nanobiotechnology 2009 7:10 doi:10.1186/1477-3155-7-10