Research

Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice

Selvaraj BarathManiKanth^1* , Kalimuthu Kalishwaralal^1* , Muthuirulappan Sriram¹ , SureshBabu Ram Kumar Pandian¹ , Hyung-seop Youn² , SooHyun Eom² and Sangiliyandi Gurunathan¹

¹  Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Anand Nagar, Krishnankoil-626190, Tamilnadu, India

²  School of Life Sciences, Systems Biology Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, South Korea

author email corresponding author email* Contributed equally

Journal of Nanobiotechnology 2010, 8:16doi:10.1186/1477-3155-8-16

Published:	14 July 2010

Abstract

Background

Oxidative stress is imperative for its morbidity towards diabetic complications, where abnormal metabolic milieu as a result of hyperglycemia, leads to the onset of several complications. A biological antioxidant capable of inhibiting oxidative stress mediated diabetic progressions; during hyperglycemia is still the need of the era. The current study was performed to study the effect of biologically synthesized gold nanoparticles (AuNPs) to control the hyperglycemic conditions in streptozotocin induced diabetic mice.

Results

The profound control of AuNPs over the anti oxidant enzymes such as GSH, SOD, Catalase and GPx in diabetic mice to normal, by inhibition of lipid peroxidation and ROS generation during hyperglycemia evidence their anti-oxidant effect during hyperglycemia. The AuNPs exhibited an insistent control over the blood glucose level, lipids and serum biochemical profiles in diabetic mice near to the control mice provokes their effective role in controlling and increasing the organ functions for better utilization of blood glucose. Histopathological and hematological studies revealed the non-toxic and protective effect of the gold nanoparticles over the vital organs when administered at dosage of 2.5 mg/kilogram.body.weight/day. ICP-MS analysis revealed the biodistribution of gold nanoparticles in the vital organs showing accumulation of AuNPs in the spleen comparatively greater than other organs.

Conclusion

The results obtained disclose the effectual role of AuNPs as an anti-oxidative agent, by inhibiting the formation of ROS, scavenging free radicals; thus increasing the anti-oxidant defense enzymes and creating a sustained control over hyperglycemic conditions which consequently evoke the potential of AuNPs as an economic therapeutic remedy in diabetic treatments and its complications.