Dose/response bars and lines showing transfection efficiency. Luciferase activity was measured (blue bars) and the corresponding straight lines generated (black lines). DOTAP NPs (25 μg DNA/mg NPs) were added at amounts of 410 ng to 16 μg NPs to 105 cells/ml (293 cells) for 48 hours. Top shows Out and In NP/DNA complexes. Bottom shows Both NP/DNA and Lip2000 (Lip) complexes; Lip/DNA complexes were added at 100 pg to 100 ng DNA.
Zhong et al. Journal of Nanobiotechnology 2010 8:6 doi:10.1186/1477-3155-8-6