Western blot analysis of purified His-ProT and His-Mre11 recombinant proteins. Elution fractions of His-ProT and His-Mre11recombinant proteins purified by 4 mg/ml SiMAG/N-NTA/Nickel magnetic matrix were subjected to SDS-PAGE. Bands were transferred to nitrocellulose membrane and specific bands were detected by antibodies directed against 6His tag by ECL system. 1-3 indicated the fractions eluted by 0.05, 0.1 and 0.25 M imidazole, respectively.
Nejadmoghaddam et al. Journal of Nanobiotechnology 2011 9:31 doi:10.1186/1477-3155-9-31