Knockdown of DCAMKL-1 and treatment with DAPT downregulates Notch-1 via miR-144. siRNA-mediated knockdown of DCAMKL-1 and DAPT treatment decreases Notch-1 (A) and HES1 mRNA (B) in HCT116 tumor xenografts. Decreased Notch-1 protein expression (brown) in NP-siDCAMKL-1, DAPT and NP-siDCAMKL-1+DAPT treated tumors compared with controls by immunohistochemical analysis (C) and by Western blot analysis (D). Knockdown of DCAMKL-1 and treatment with DAPT results in increased expression of pri-miR-144 miRNA in tumor xenografts (E) and decreases luciferase activity (luciferase units) following transfection with plasmid-encoding luciferase containing the miR-144 binding site in HCT116 cells. For bar graph in A, B, E and F, values are given as average ± SEM, and asterisks denote statistically significant differences (P < 0.01) compared with control (NP alone).
Sureban et al. Journal of Nanobiotechnology 2011 9:40 doi:10.1186/1477-3155-9-40