Immunohistochemical staining of apoptotic cells in HCC827 xenograft tumors. (A) TUNEL staining was performed on tissue sections of HCC827 xenograft tumors treated as follows: (a) group 1, control group; (b) group 2, 50 μmol/kg ruthenium (II)-arene complex treatment; (c) group 3, 100 μmol/kg ruthenium (II)-arene complex treatment. Arrows: TUNEL positive cells (indicate apoptotic cells). Scale bars: 50 μm. (d) Quantitative analysis of apoptotic cells after various treatments shown in (Figure 5. A a, b, c). (B) Inhibition of tumor growth in HCC827 nude mice with different treatments. (a) The different treatment effects on the tumor growth inhibition in nude mice inoculated with HCC827 cells: group 1, no treatment, serve as a control group; group 2, 50 μmol/kg ruthenium (II)-arene complex treatment; group 3, 100 μmol/kg ruthenium (II)-arene complex. (b) Quantitative analysis of tumor volume after various treatments shown in (Figure 5. B a). Each data represents the mean ± standard deviation (n = 7). *Indicates significant difference in comparison to the control group (p < 0.05).
Zhang et al. Journal of Nanobiotechnology 2011 9:6 doi:10.1186/1477-3155-9-6